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A mutant spacer sequence between -35 and -10 elements makes the P(lac) promoter hyperactive and cAMP receptor protein-independent

To determine whether the spacer region between the -35 and -10 elements plays any sequence-specific role, we randomized the GC-rich sequence ((-20)CCGGCTCG(-13)) within the spacer region of the cAMP-dependent lac promoter and selected an activator-independent mutant, which showed extraordinarily hig...

詳細記述

保存先:
書誌詳細
主要な著者: Liu, Mofang, Tolstorukov, Michael, Zhurkin, Victor, Garges, Susan, Adhya, Sankar
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2004
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC406441/
https://ncbi.nlm.nih.gov/pubmed/15118087
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401929101
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