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A mutant spacer sequence between -35 and -10 elements makes the P(lac) promoter hyperactive and cAMP receptor protein-independent
To determine whether the spacer region between the -35 and -10 elements plays any sequence-specific role, we randomized the GC-rich sequence ((-20)CCGGCTCG(-13)) within the spacer region of the cAMP-dependent lac promoter and selected an activator-independent mutant, which showed extraordinarily hig...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
2004
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC406441/ https://ncbi.nlm.nih.gov/pubmed/15118087 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401929101 |
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