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Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region

BACKGROUND: The success of PCR technique depends on many factors, such as high quality DNA pellets obtained from blood samples, good reagents and adequate conditions of amplification. Taking these limitations into account, a retrospective epidemiological study for malaria diagnosis was conducted in...

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Detalhes bibliográficos
Main Authors: Scopel, Kézia KG, Fontes, Cor JF, Nunes, Álvaro C, Horta, Maria de Fátima, Braga, Érika M
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC406413/
https://ncbi.nlm.nih.gov/pubmed/15056392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2875-3-8
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