Assessment of RainDrop BS-seq as a method for large-scale, targeted bisulfite sequencing

We present a systematic assessment of RainDrop BS-seq, a novel method for large-scale, targeted bisulfite sequencing using microdroplet-based PCR amplification coupled with next-generation sequencing. We compared DNA methylation levels at 498 target loci (1001 PCR amplicons) in human whole blood, os...

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Detalhes bibliográficos
Main Authors: Paul, Dirk S, Guilhamon, Paul, Karpathakis, Anna, Butcher, Lee M, Thirlwell, Christina, Feber, Andrew, Beck, Stephan
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2014
Assuntos:
Brief Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4063826/
https://ncbi.nlm.nih.gov/pubmed/24518816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/epi.28041
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