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Facile Bench-Top Fabrication of Enclosed Circular Microchannels Provides 3D Confined Structure for Growth of Prostate Epithelial Cells

We present a simple bench-top method to fabricate enclosed circular channels for biological experiments. Fabricating the channels takes less than 2 hours by using glass capillaries of various diameters (from 100 µm up to 400 µm) as a mould in PDMS. The inner surface of microchannels prepared in this...

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Detalhes bibliográficos
Main Authors: Dolega, Monika E., Wagh, Jayesh, Gerbaud, Sophie, Kermarrec, Frederique, Alcaraz, Jean-Pierre, Martin, Donald K., Gidrol, Xavier, Picollet-D’hahan, Nathalie
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4063722/
https://ncbi.nlm.nih.gov/pubmed/24945245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0099416
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