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Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis
Extracellular flux (XF) analysis has become a mainstream method to measure bioenergetic function in cells and tissues. While this technique is commonly used to measure energetics in intact cells, we outline here a detailed XF protocol for measuring respiration in permeabilized cells. Cells are perme...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2014
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4063296/ https://ncbi.nlm.nih.gov/pubmed/24457333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.018 |
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