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Time-Resolved Fluorescence Spectroscopy Measures Clustering and Mobility of a G Protein-Coupled Receptor Opsin in Live Cell Membranes

[Image: see text] Determining membrane protein quaternary structure is extremely challenging, especially in live cell membranes. We measured the oligomerization of opsin, a prototypical G protein-coupled receptor with pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCC...

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Detalhes bibliográficos
Main Authors: Comar, William D., Schubert, Sarah M., Jastrzebska, Beata, Palczewski, Krzysztof, Smith, Adam W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4063175/
https://ncbi.nlm.nih.gov/pubmed/24831851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja501948w
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