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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents

INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...

詳細記述

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書誌詳細
主要な著者: Goldschmidt, Pablo, Degorge, Sandrine, Merabet, Lilia, Chaumeil, Christine
フォーマット: Artigo
言語:Inglês
出版事項: Public Library of Science 2014
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061000/
https://ncbi.nlm.nih.gov/pubmed/24936792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094886
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