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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents

INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...

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Bibliografski detalji
Glavni autori: Goldschmidt, Pablo, Degorge, Sandrine, Merabet, Lilia, Chaumeil, Christine
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2014
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061000/
https://ncbi.nlm.nih.gov/pubmed/24936792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094886
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