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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents

INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...

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Bibliografiset tiedot
Päätekijät: Goldschmidt, Pablo, Degorge, Sandrine, Merabet, Lilia, Chaumeil, Christine
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Public Library of Science 2014
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061000/
https://ncbi.nlm.nih.gov/pubmed/24936792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094886
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