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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents

INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...

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Autors principals: Goldschmidt, Pablo, Degorge, Sandrine, Merabet, Lilia, Chaumeil, Christine
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061000/
https://ncbi.nlm.nih.gov/pubmed/24936792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094886
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