Chemical reactivation of quenched fluorescent protein molecules enables resin-embedded fluorescence microimaging

Resin embedding is a well-established technique to prepare biological specimens for microscopic imaging. However, it is not compatible with modern green-fluorescent protein (GFP) fluorescent-labelling technique because it significantly quenches the fluorescence of GFP and its variants. Previous empi...

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Autori principali: Xiong, Hanqing, Zhou, Zhenqiao, Zhu, Mingqiang, Lv, Xiaohua, Li, Anan, Li, Shiwei, Li, Longhui, Yang, Tao, Wang, Siming, Yang, Zhongqin, Xu, Tonghui, Luo, Qingming, Gong, Hui, Zeng, Shaoqun
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Pub. Group 2014
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4059927/
https://ncbi.nlm.nih.gov/pubmed/24886825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms4992
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