Comparison and Validation of Some ITS Primer Pairs Useful for Fungal Metabarcoding Studies

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amplification and sequencing of relatively short DNA regions. For fungi, the internal transcribed spacer (ITS) region in the ribosomal RNA (rRNA) operon has been accepted as the formal fungal barcode. De...

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Main Authors: Op De Beeck, Michiel, Lievens, Bart, Busschaert, Pieter, Declerck, Stéphan, Vangronsveld, Jaco, Colpaert, Jan V.
格式: Artigo
語言:Inglês
出版: Public Library of Science 2014
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4059633/
https://ncbi.nlm.nih.gov/pubmed/24933453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0097629
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