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Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
Herein, we describe a protocol for the isolation of human embryonic stem cells (hESCs)-derived vascular cells at various stages of development. The cells are isolated from 10 to 15-d-old human embryoid bodies (EBs) cultured in suspension. After dissociation, cells are labeled with anti-CD34 or anti-...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4059065/ https://ncbi.nlm.nih.gov/pubmed/20539287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.31 |
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