Access of Hydrogen-Radicals to the Peptide-Backbone as a Measure for Estimating the Flexibility of Proteins Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A factor for estimating the flexibility of proteins is described that uses a cleavage method of “in-source decay (ISD)” coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The MALDI-ISD spectra of bovine serum albumin (BSA), myoglobin and thioredoxin show discontin...

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Bibliographic Details
Main Authors: Takayama, Mitsuo, Nagoshi, Keishiro, Iimuro, Ryunosuke, Inatomi, Kazuma
Format: Artigo
Language:Inglês
Published: Molecular Diversity Preservation International (MDPI) 2014
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4057740/
https://ncbi.nlm.nih.gov/pubmed/24828203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms15058428
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