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ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions

Chromatin immunoprecipitation (ChIP) allows specific protein-DNA interactions to be isolated. Combining ChIP with high-throughput sequencing reveals the DNA sequence involved in these interactions. Here, we describe how to perform ChIP-seq starting with whole tissues or cell lines, and ending with m...

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Bibliografiske detaljer
Main Authors: Schmidt, Dominic, Wilson, Michael D., Spyrou, Christiana, Brown, Gordon D., Hadfield, James, Odom, Duncan T.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4052679/
https://ncbi.nlm.nih.gov/pubmed/19275939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2009.03.001
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