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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
Working with delicate tissue can be a complicating factor when performing immunohistochemical assessment. Here, we present a method that utilizes a ring-supported hydrophilized PTFE membrane to provide structural support to both living and fixed tissue during immunohistochemical processing, which al...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4048354/ https://ncbi.nlm.nih.gov/pubmed/24379013 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51018 |
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