Amplification of Coccidioidal DNA in Clinical Specimens by PCR

Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.

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Bibliografiske detaljer
Main Authors: Johnson, Suzanne M., Simmons, Keira A., Pappagianis, Demosthenes
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2004
Fag:
Mycology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC404645/
https://ncbi.nlm.nih.gov/pubmed/15131158
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.5.1982-1985.2004
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