Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells

Fluorescence microscopy of GFP-tagged proteins is a fundamental tool in cell biology, but without seeing the structure of the surrounding cellular space, functional information can be lost. Here we present a protocol that preserves GFP and mCherry fluorescence in mammalian cells embedded in resin wi...

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Main Authors: Peddie, Christopher J., Blight, Ken, Wilson, Emma, Melia, Charlotte, Marrison, Jo, Carzaniga, Raffaella, Domart, Marie-Charlotte, O׳Toole, Peter, Larijani, Banafshe, Collinson, Lucy M.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2014
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4045205/
https://ncbi.nlm.nih.gov/pubmed/24637200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ultramic.2014.02.001
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