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Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development.

Infectious human respiratory syncytial virus (RSV) was produced by the intracellular coexpression of five plasmid-borne cDNAs. One cDNA encoded a complete positive-sense version of the RSV genome (corresponding to the replicative intermediate RNA or antigenome), and each of the other four encoded a...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Collins, P L, Hill, M G, Camargo, E, Grosfeld, H, Chanock, R M, Murphy, B R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1995
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40442/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8524804/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.25.11563
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