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Bacteriophage-Based Genetic System for Selection of Nonsplicing Inteins
A genetic selection system that detects splicing and nonsplicing activities of inteins was developed based on the ability to rescue a T4 phage strain with a conditionally inactive DNA polymerase. This phage defect can be complemented by expression of plasmid-encoded phage RB69 DNA polymerase. Insert...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2004
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC404401/ https://ncbi.nlm.nih.gov/pubmed/15128583 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.5.3158-3162.2004 |
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