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iRFP Is a Real Time Marker for Transformation Based Assays in High Content Screening

Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible with...

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Detalhes bibliográficos
Main Authors: Paulus-Hock, Viola, Cheung, Eric C., Roxburgh, Patricia, Vousden, Karen H., Hock, Andreas K.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4041769/
https://ncbi.nlm.nih.gov/pubmed/24887316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0098399
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