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Direct Sequencing from the Minimal Number of DNA Molecules Needed to Fill a 454 Picotiterplate

The large amount of DNA needed to prepare a library in next generation sequencing protocols hinders direct sequencing of small DNA samples. This limitation is usually overcome by the enrichment of such samples with whole genome amplification (WGA), mostly by multiple displacement amplification (MDA)...

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Bibliografske podrobnosti
Main Authors: Džunková, Mária, Garcia-Garcerà, Marc, Martínez-Priego, Llúcia, D’Auria, Giussepe, Calafell, Francesc, Moya, Andrés
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4041646/
https://ncbi.nlm.nih.gov/pubmed/24887077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0097379
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