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Single-molecule high-resolution imaging with photobleaching

Conventional light microscopy is limited in its resolving power by the Rayleigh limit to length scales on the order of 200 nm. On the other hand, spectroscopic techniques such as fluorescence resonance energy transfer cannot be used to measure distances >10 nm, leaving a “gap” in the ability of o...

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Autori principali: Gordon, Matthew P., Ha, Taekjip, Selvin, Paul R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC404067/
https://ncbi.nlm.nih.gov/pubmed/15096603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401638101
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