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Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein–intein junction

Protein splicing is a posttranslational autocatalytic process in which an intervening sequence, termed an intein, is removed from a host protein, the extein. Although we have a reasonable picture of the basic chemical steps in protein splicing, our knowledge of how these are catalyzed and regulated...

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Detalhes bibliográficos
Main Authors: Romanelli, Alessandra, Shekhtman, Alexander, Cowburn, David, Muir, Tom W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC404056/
https://ncbi.nlm.nih.gov/pubmed/15087498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0306616101
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