Optimization and Validation of the TZM-bl Assay for Standardized Assessments of Neutralizing Antibodies Against HIV-1

The TZM-bl assay measures antibody-mediated neutralization of HIV-1 as a function of reductions in HIV-1 Tat-regulated firefly luciferase (Luc) reporter gene expression after a single round of infection with Env-pseudotyped viruses. This assay has become the main endpoint neutralization assay used f...

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Main Authors: Sarzotti-Kelsoe, Marcella, Bailer, Robert T, Turk, Ellen, Lin, Chen-li, Bilska, Miroslawa, Greene, Kelli M., Gao, Hongmei, Todd, Christopher A., Ozaki, Daniel A., Seaman, Michael S., Mascola, John R., Montefiori, David C.
Format: Artigo
Sprog:Inglês
Udgivet: 2013
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4040342/
https://ncbi.nlm.nih.gov/pubmed/24291345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2013.11.022
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