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Cloning, Expression and Characterization of a Lipase Encoding Gene from Human Oral Metagenome
The human oral metagenomic DNA cloned into plasmid pUC19 was used to construct a DNA library in Escherichia coli. Functional screening of 40,000 metagenomic clones led to identification of a clone LIP2 that exhibited halo on tributyrin agar plate. Sequence analysis of LIP2 insert DNA revealed a 939 ...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer India
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4039722/ https://ncbi.nlm.nih.gov/pubmed/24891735 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-014-0455-y |
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