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Technicalities and Glitches of Terminal Restriction Fragment Length Polymorphism (T-RFLP)

Terminal restriction fragment length polymorphism (T-RFLP) is a rapid, robust, inexpensive and simple tool for microbial community profiling. Methods used for DNA extraction, PCR amplification and digestion of amplified products have a considerable impact on the results of T-RFLP. Pitfalls of the me...

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Hlavní autoři: Prakash, Om, Pandey, Prashant K., Kulkarni, Girish J., Mahale, Kiran N., Shouche, Yogesh S.
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer India 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4039717/
https://ncbi.nlm.nih.gov/pubmed/24891731
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-014-0461-0
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