E. coli Selection of Human Genes Encoding Secreted and Membrane Proteins Based on cDNA Fusions to a Leaderless β-Lactamase Reporter

Although several signal peptide-trapping methods have been devised and used to detect signal sequences, none have relied on using E.coli to identify eukaryotic proteins with signal peptides. Here, we describe a system for selecting human secreted and membrane proteins in E. coli followed by the dire...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Tan, Ruoying, Jiang, Xin, Jackson, Alan, Jin, Pei, Yang, Junming, Lee, Ernestine, Duggan, Brendan, Stuve, Laura L., Fu, Glenn K.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Cold Spring Harbor Laboratory Press 2003
Gaiak:
Methods
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC403786/
https://ncbi.nlm.nih.gov/pubmed/12869575
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.1000903
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