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High ionic strength narrows the population of sites participating in protein ion-exchange adsorption: A single-molecule study
The retention and elution of proteins in ion-exchange chromatography is routinely controlled by adjusting the mobile phase salt concentration. It has repeatedly been observed, as judged from adsorption isotherms, that the apparent heterogeneity of adsorption is lower at more-eluting, higher ionic st...
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| Main Authors: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4035562/ https://ncbi.nlm.nih.gov/pubmed/24751557 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2014.03.075 |
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