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Distance determination in proteins using designed metal ion binding sites and site-directed spin labeling: application to the lactose permease of Escherichia coli.

As shown in the accompanying paper, the magnetic dipolar interaction between site-directed metal-nitroxide pairs can be exploited to measure distances in T4 lysozyme, a protein of known structure. To evaluate this potentially powerful method for general use, particularly with membrane proteins that...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Voss, J, Hubbell, W L, Kaback, H R
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40344/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8618889/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.26.12300
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