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Mapping the miRNA interactome by crosslinking ligation and sequencing of hybrids (CLASH)

RNA-RNA interactions play critical roles in many cellular processes but studying them is difficult and laborious. Here, we describe an experimental procedure, termed crosslinking ligation and sequencing of hybrids (CLASH), which allows high-throughput identification of sites of RNA-RNA interaction....

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Detalhes bibliográficos
Main Authors: Helwak, Aleksandra, Tollervey, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4033841/
https://ncbi.nlm.nih.gov/pubmed/24577361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.043
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