Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase

Malate dehydrogenase (MDH) plays important metabolic roles in bacteria. In this study, the recombinant MDH protein (His-MDH) of Brucella abortus was purified and its ability to catalyze the conversion of oxaloacetate (OAA) to L-malate (hereon referred to as MDH activity) was analyzed. Michaelis Cons...

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Detalhes bibliográficos
Main Authors: Han, Xiangan, Tong, Yongliang, Tian, Mingxing, Zhang, Yuxi, Sun, Xiaoqing, Wang, Shaohui, Qiu, Xusheng, Ding, Chan, Yu, Shengqing
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4033508/
https://ncbi.nlm.nih.gov/pubmed/24895685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/973751
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