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Conversion of a Rhizopus chinensis lipase into an esterase by lid swapping

In an effort to explore the feasibility of converting a lipase into an esterase by modifying the lid region, we designed and characterized two novel Rhizopus chinensis lipase variants by lid swapping. The substrate specificity of an R. chinensis lipase was successfully modified toward water-soluble...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Yu, Xiao-Wei, Zhu, Shan-Shan, Xiao, Rong, Xu, Yan
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2014
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4031936/
https://ncbi.nlm.nih.gov/pubmed/24670990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.M043950
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