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A novel approach to the generation of seamless constructs for plant transformation
BACKGROUND: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone or in combina...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2014
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4030040/ https://ncbi.nlm.nih.gov/pubmed/24855486 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-10-10 |
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