A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus

A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1−ORF2 junction of MNV. The broad reactivit...

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Detalhes bibliográficos
Main Authors: Hanaki, Ken-Ichi, Ike, Fumio, Kajita, Ayako, Yasuno, Wataru, Yanagiba, Misato, Goto, Motoki, Sakai, Kouji, Ami, Yasushi, Kyuwa, Shigeru
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4029972/
https://ncbi.nlm.nih.gov/pubmed/24847970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0098108
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