Sensitivity of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral blood

OBJECTIVE: To evaluate the effectiveness of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral blood samples. METHODS: DNA extraction was performed using Promega Wizard® Genomic kits. PCR employing RV1/RV2 primers yielded 145-bp amplicons. Real-time PCR was perf...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: da Costa Lima, Manoel Sebastião, Zorzenon, Denielly Christina Rodrigues, Dorval, Maria Elizabeth Cavalheiros, Pontes, Elenir Rose Jardim Cury, Oshiro, Elisa Teruya, Cunha, Rodrigo, Andreotti, Renato, Matos, Maria de Fatima Cepa
Format: Artigo
Langue:Inglês
Publié: Asian Pacific Tropical Medicine Press 2013
Sujets:
Basic Researches
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4027260/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S2222-1808(13)60003-1
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