PDZ Affinity Chromatography: A general method for affinity purification of proteins based on PDZ domains and their ligands

PDZ (PSD-95, DiscsLarge, ZO1) domains function in nature as protein binding domains within scaffold and membrane-associated proteins. They comprise ~ 90 residues and make specific, high affinity interactions with complementary C-terminal peptide sequences, with other PDZ domains, and with phospholip...

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Bibliografiske detaljer
Main Authors: Walkup, Ward G., Kennedy, Mary B.
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4024478/
https://ncbi.nlm.nih.gov/pubmed/24607360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2014.02.015
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