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Nuclear Microinjection to Assess How Heterologously Expressed Proteins Impact Ca(2+) Signals in Xenopus Oocytes
The Xenopus oocyte is frequently used for heterologous expression and for studying the spatiotemporal patterning of Ca(2+) signals. Here, we outline a protocol for nuclear microinjection of the Xenopus oocyte for the purpose of studying how subsequently expressed proteins impact intracellular Ca(2+)...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4021487/ https://ncbi.nlm.nih.gov/pubmed/23457340 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot072785 |
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