Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid.

We report here the reconstruction of a functional linear replicon, the 12-kilobase Streptomyces clavuligerus plasmid pSCL, from separate DNA fragments cloned in Escherichia coli on the pUC19 plasmid. Protein-free DNA molecules containing the full-length pSCL sequence, an internally inserted thiostre...

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Main Authors: Shiffman, D, Cohen, S N
Format: Artigo
Jezik:Inglês
Izdano: 1992
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC402135/
https://ncbi.nlm.nih.gov/pubmed/1631099
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