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Characterization of the temperature-sensitive reaction of F(1)-ATPase by using single-molecule manipulation

F(1)-ATPase (F(1)) is a rotary motor protein that couples ATP hydrolysis to mechanical rotation with high efficiency. In our recent study, we observed a highly temperature-sensitive (TS) step in the reaction catalyzed by a thermophilic F(1) that was characterized by a rate constant remarkably sensit...

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Detalhes bibliográficos
Main Authors: Watanabe, Rikiya, Noji, Hiroyuki
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4019956/
https://ncbi.nlm.nih.gov/pubmed/24825532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04962
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