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Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis

There is significant interest in the use of primary intestinal epithelial cells in monolayer culture to model intestinal biology. However, it has proven to be challenging to create functional, differentiated monolayers using current culture methods, likely due to the difficulty in expanding these ce...

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Bibliographic Details
Main Authors: Moon, Clara, VanDussen, Kelli L., Miyoshi, Hiroyuki, Stappenbeck, Thaddeus S.
Format: Artigo
Language:Inglês
Published: 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4019725/
https://ncbi.nlm.nih.gov/pubmed/24220295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mi.2013.98
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