Binding of the sigma 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting.

We have used a nonspecific protein cleaving reagent to map the interactions between subunits of the multisubunit enzyme RNA polymerase (Escherichia coli). We developed suitable conditions for using an untethered Fe-EDTA reagent, which does not bind significantly to proteins. Comparison of the cleave...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Greiner, D P, Hughes, K A, Gunasekera, A H, Meares, C F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40180/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8552678/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.1.71
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