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A defective proton pump, point-mutated bacteriorhodopsin Asp96----Asn is fully reactivated by azide.

Addition of azide fully restored the proton pump activity of defective bacteriorhodopsin (BR) mutant protein Asp96----Asn. The decay time of M of BR Asp96----Asn, the longest living intermediate, was decreased from 500 ms at pH 7.0 to approximately 1 ms under conditions of saturating azide concentra...

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Détails bibliographiques
Publié dans:EMBO J
Auteurs principaux: Tittor, J, Soell, C, Oesterhelt, D, Butt, H J, Bamberg, E
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 1989
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC401504/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2555165/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb08512.x
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