A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms

Shiga toxin-producing E. coli (STEC), of various serogroups harboring the intimin gene, form a serious threat to human health. They are asymptomatically carried by cattle. In this study, a quantitative real-time PCR (qPCR) method was developed as a molecular method to detect and quantify Shiga toxin...

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Auteurs principaux: Verstraete, Karen, Van Coillie, Els, Werbrouck, Hadewig, Van Weyenberg, Stephanie, Herman, Lieve, Del-Favero, Jurgen, De Rijk, Peter, De Zutter, Lieven, Joris, Maria-Adelheid, Heyndrickx, Marc, De Reu, Koen
Format: Artigo
Langue:Inglês
Publié: MDPI 2014
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4014729/
https://ncbi.nlm.nih.gov/pubmed/24681714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/toxins6041201
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