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A cell free system for functional centromere and kinetochore assembly Authors
This protocol describes a cell free system to study vertebrate centromere and kinetochore formation. We reconstitute tandem arrays of centromere protein A (CENP-A) nucleosomes as a substrate for centromere and kinetochore assembly. These chromatin substrates are immobilized on magnetic beads and the...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4011387/ https://ncbi.nlm.nih.gov/pubmed/23018190 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.112 |
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