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Optimizing stem-loop qPCR assays through multiplexed cDNA synthesis of U6 and miRNAs
We recently reported that hairpin (or stem-loop) priming is better-suited than polyA tailing to generate cDNA for plant microRNA qPCR. One major limitation of this method is the need to perform individual cDNA synthesis reactions for the reference gene and test miRNAs. Here, we report a novel fusion...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4010539/ https://ncbi.nlm.nih.gov/pubmed/23673353 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/psb.24918 |
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