In vitro synthesis of vertebrate U1 snRNA.
We have developed a DNA-dependent in vitro transcription system for vertebrate snRNA genes. By isolating the nuclei (germinal vesicles, GVs) of Xenopus laevis oocytes under oil to maintain the in vivo composition of their internal milieu, we are able to prepare nuclei that retain their ability to sy...
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| Veröffentlicht in: | EMBO J |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
1989
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400801/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2714253/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb03375.x |
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