A New Direct Single-Molecule Observation Method for DNA Synthesis Reaction Using Fluorescent Replication Protein A

Using a single-stranded region tracing system, single-molecule DNA synthesis reactions were directly observed in microflow channels. The direct single-molecule observations of DNA synthesis were labeled with a fusion protein consisting of the ssDNA-binding domain of a 70-kDa subunit of replication p...

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Bibliographische Detailangaben
Hauptverfasser: Takahashi, Shunsuke, Kawasaki, Shohei, Miyata, Hidefumi, Kurita, Hirofumi, Mizuno, Takeshi, Matsuura, Shun-ichi, Mizuno, Akira, Oshige, Masahiko, Katsura, Shinji
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2014
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4003986/
https://ncbi.nlm.nih.gov/pubmed/24625741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s140305174
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