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Optimised concentration and purification of retroviruses using membrane chromatography

The ability of an anion exchange membrane to purify a γ-retrovirus was assessed and optimised with respect to different loading and wash buffers. Recoveries of infectious virus greater than 50% were consistently obtained, while specific titre was increased up to one thousand fold when compared to th...

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Autors principals: McNally, D.J., Darling, D., Farzaneh, F., Levison, P.R., Slater, N.K.H.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4003387/
https://ncbi.nlm.nih.gov/pubmed/24685165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2014.03.023
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