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Optimised concentration and purification of retroviruses using membrane chromatography
The ability of an anion exchange membrane to purify a γ-retrovirus was assessed and optimised with respect to different loading and wash buffers. Recoveries of infectious virus greater than 50% were consistently obtained, while specific titre was increased up to one thousand fold when compared to th...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier
2014
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4003387/ https://ncbi.nlm.nih.gov/pubmed/24685165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2014.03.023 |
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